An in vitro assay for compounds toxic to rumen protozoa
نویسندگان
چکیده
منابع مشابه
Rumen Protozoa - Potential for Manipulation
The manipulation of rumen function through the removal of the ciliate protozoa has not resulted in consistent changes in ruminant production. Current research findings are discussed with particular emphasis given to the role of protozoa in fibre digestion in the rumen and the availability of protein for intestinal digestion. Dietary conditions under which positive responses to defaunation can b...
متن کاملRumen holotrich ciliate protozoa.
Factors Influencing Size of the Protozoal Population .................................................................. 28 Diurnal Variations in the Holotrich Population ............................................ ......................28 IN VITRO CULTIVATION OF HOLOTRICH PROTOZOA...........................................................28 TECHNIQUES FOR ISOLATION OF HOLOTRICH CILIATES FROM R...
متن کاملContribution of protozoa to lysine synthesis in the in vitro rumen microbial ecosystem.
Isotopic tracer experiments were conducted in vitro to determine contribution of protozoa toward the biosynthesis of lysine in the rumen microbial ecosystem. The presence of protozoa in a rumen microbial suspension always increased lysine synthesis from aspartate. Rumen contents from a faunated goat produced a higher amount of lysine than did those from a defaunated one.
متن کاملProtease activities of rumen protozoa.
Intact, metabolically active rumen protozoa prepared by gravity sedimentation and washing in a mineral solution at 10 to 15 degrees C had comparatively low proteolytic activity on azocasein and low endogenous proteolytic activity. Protozoa washed in 0.1 M potassium phosphate buffer (pH 6.8) at 4 degrees C and stored on ice autolysed when they were warmed to 39 degrees C. They also exhibited low...
متن کاملAutomated High-Content Assay for Compounds Selectively Toxic to Trypanosoma cruzi in a Myoblastic Cell Line
BACKGROUND Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, represents a very important public health problem in Latin America where it is endemic. Although mostly asymptomatic at its initial stage, after the disease becomes chronic, about a third of the infected patients progress to a potentially fatal outcome due to severe damage of heart and gut tissues. There is an urgent...
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ژورنال
عنوان ژورنال: New Zealand Journal of Agricultural Research
سال: 1982
ISSN: 0028-8233,1175-8775
DOI: 10.1080/00288233.1982.10425218